Non-radiative Excitation Fluorescence Microscopy
Proceeding of SPIE on Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIII, San Francisco
Förster Resonance Energy Transfer (FRET) is a powerful tool for studying cellular processes. It can also be used to improve the axial resolution of fluorescence microscope by coating the substrate with the donor species (quantum dots). We propose a new method, which prevent dewetting of the thin film of donors, making long time observation possible. Hence, quantum dots are covalently bound to one side of the substrate using a silanisation process. Atomic force microscopy and ellipsometry were used to characterize the substrate. Further experiments with Giant Unilamellar Vesicles were conducted to investigate steric and undulation forces occurring in membrane-surface interactions.