Authors
Dalia El Arawi, Marcelina Cardoso Dos Santos, Cyrille Vézy, Rodolphe Jaffiol,
Title
Incidence angle calibration for prismless total internal reflection fluorescence microscopy
In
Optics Letters
Volume
44
Issue
7
Pages
1710–1713
Publisher
OSA
Year
2019
Indexed by
Abstract
We propose a calibration routine useful to evaluate the in- cident angle in total internal reflection fluorescence (TIRF) microscopy. This procedure is based on critical angle mea- surements conducted in the back focal plane (BFP) of the objective. Such BFP imaging can be easily implemented on any TIRF setup, making this technique very attractive. Calibration exactitude was demonstrated by comparing the theoretical angular dependence of the electric field intensity at glass/water interface to experimental observations.
Affiliations
Offprint