Authors
Catalina David, Nicolas Guillot, Hong Shen, Timothée Toury, Marc Lamy de la Chapelle,
Title
SERS detection of biomolecules using lithographed nanoparticles towards a reproducible SERS biosensor
In
Nanotechnology
Volume
21
Issue
47
Pages
475501
Publisher
IOP
Year
2010
Publisher's URL
http://stacks.iop.org/0957-4484/21/i=47/a=475501
Indexed by
Abstract
In this paper we highlight the accurate spectral detection of bovine serum albumin and ribonuclease-A using a surface-enhanced Raman scattering (SERS) substrate based on gold nanocylinders obtained by electron-beam lithography (EBL). The nanocylinders have diameters from 100 to 180 nm with a gap of 200 nm. We demonstrate that optimizing the size and the shape of the lithographed gold nanocylinders, we can obtain SERS spectra of proteins at low concentration. This SERS study enabled us to estimate high enhancement factors (10 5 for BSA and 10 7 for RNase-A) of important bands in the protein Raman spectrum measured for 1 mM concentration. We demonstrate that, to reach the highest enhancement, it is necessary to optimize the SERS signal and that the main parameter of optimization is the LSPR position. The LSPR have to be suitably located between the laser excitation wavelength, which is 632.8 nm, and the position of the considered Raman band. Our study underlines the efficiency of gold nanocylinder arrays in the spectral detection of proteins.
Affiliations
Offprint