Yubing Pu, Bertrand Laratte, Robert S. Marks, Elena Rodica Ionescu,
Toxicity of pesticides and nanoparticles to neutrophils
4th International Conference on Safe production and use of Nanomaterials, Grenoble, France, November 18-20
Indexed by
While the toxicity and ecotoxicity of herbicide, pesticide and nanoparticles have been reported over the years, the investigation of direct interactions between such chemical compounds and neutrophils have rarely been reported. In the present work, neutrophils cells were extracted from porcine whole blood and used to investigate the toxicity of different classes of toxicants that humans may contact either through the water resources or through in air. Thus, the influences of atrazine, carbofuran, Dimethyl sulfoxide (DMSO) and three types of nanoparticles (NPs) with similar sizes, aluminium oxyde NPs (< 50 nm), copper NPs (< 100 nm) and nickel NPs (< 100 nm) on neutrophils have been studied using “in vitro” chemiluminescence experiments. Experimentally, several concentrations of toxicants dissolved or suspended in water or DMSO were incubated with neutrophils for different periods of time. The survival rate and morphology of neutrophils were observed using optical microscope and scanning electron microscope, respectively. Interestingly, it has been found that results are highly dependent on several parameters such as the donor of the blood, type of toxicant, evaporation of aqueous solution from the microtiter plate wells, temperature etc. Moreover, the interaction time between toxicant content and cells played a significant role in the evolution of chemiluminescent signal kinetics. It was also founded that insoluble toxicants (nanomaterials) had stronger effects on neutrophils cells when compared to the soluble chemicals (atrazine, carbofuran and DMSO). Additionally, the experimental results are expected to be compared with the modeling outcome performed by USEtox methodology which is considered as a scientific consensus to assess the toxicity of a product.